Quality control quality system waisman biomanufacturing. Performance validation of the bd accuri c6 was then done using 6 and 8peak fluorescent bead mixtures spherotech according to instructions bd accuri c6 software user guide. Bd accuri c6 flow cytometer instrument manual 782001801 rev2 1 the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Technologies have developed, and reading accuri c6 user manual printable 2019 books might be easier and simpler. Accuri c6 flow cytometer instrument manual about service. Designed from the ground up to be compact and easy to use, the bd accuri c6.
Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. Bd accuri c6 plus system users guide bd accuri c6 plus system self installation guide. Bd accuri c6 software will display this notification based on whichever trigger is reached first. Accuri cytometers c6 flow cytometer community, manuals. The bd csampler will auto matically align itself and then remain in the home position figure 8 9. Even new flow cytometry users find it so easy to learn and use that. Accuri c6 is an easy to use flow cytometer which can be connected to an autosampler 48 and 96 well, and 24 tube rack, we do not have one. Cflow user guide 782001101 revd 1 1 introduction to cflow the accuri cflow or cflow plus software cflow allows you to control the c6 flow cytometer system to acquire data, generate statistics, and analyze results. Turn on the computer and log in with your ucf info. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. Accuri c6 flow cytometer instrument manual about service yumpu. An automated notification and reminder system is contained in the bd accuri c6 software to alert the user to perform the recommended preventive maintenance as described in this manual. Analyzing one cell at a time in this episode, i present to you, maybe the best entry level flow cytometer in the market, bd accuri c6.
Standard fluorochrome in use are identical to facs calibur. Bd biosciences bd accuri c6 plus community, manuals and. Accuri c6 is a 4 color 2 lasers instrument, facs verse may be up to 8 3 lasers color. Bd accuri c6 plus flow cytometer technical specifications tools. Only operate the accuri c6 flow cytometer with an original ed version of software.
Bd accuri c6 plus software features an intuitive user interface. The user control the speed of the sample uptake and can chose the optimal core size for their sample. Cord black usb cable c6 flow cytometer instruction manual cflow software cd cflow quick. Bd accuri c6 flow cytometer instrument manual 7820018 rev4 1 1 introduction the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Do not use cflow until the c6 cytometer has been completely set up see the c6. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. Assemble the computer according to the manufacturers instructions. Plate setup for ending a data collection session 80. Designed from the ground up to be compact and easy to use, the bd accuri c6 flow cytometer fits in any lab and is readily accessible. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11.
Deluca biochemical sciences building 440 henry mall madison, wi 53706. Free download books accuri c6 user manual printable 2019 we all know that reading accuri c6 user manual printable 2019 is helpful, because we are able to get enough detailed information online from your resources. There youll find a note with the following message do not use. This includes removal and replacement of the peristaltic pump tubing, inline sheath filter and the fluidic bottle filters. You can also set custom speedscore sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. Bd accuri c6 plus flow cytometer with its compact 11 x 14. Free download books accuri c6 user manual printable 2019 you know that reading accuri c6 user manual printable 2019 is helpful, because we could get enough detailed information online from the resources. Bd accuri c6 plus compensation calculator microsoft excel bd accuri c6 plus fluorochromedye reference chart. How to do compensation in bd accuri c6 website url. Since yeasts are relatively large i use an accuri c6 to count bacteria so yeast are large to me i would go with the fasterlarger core size. The quality control department at waisman biomanufacturing is staffed by highly qualified personnel who work to provide a comprehensive range of analytical testing services that ensures product quality and consistency during all stages of development and manufacturing. Using the validation beads provided by accuri, run 50,000 events from each tube on the slow setting and save the data as todays date bead file. Accuri c6 maintenance cleaning and decontamination.
Accuri c6 user guide accuri c6 user guide if you ally infatuation such a referred accuri c6 user guide books that will come up with the money for you worth, get the very best seller from us currently from several preferred authors. The bd accuri c6 plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the singlecell level. Technology has developed, and reading accuri c6 user manual printable 2019 books could be far more convenient and easier. So the choice should be addressed by the kind of flow experiments you plan to do. Service for bd accuri c6 systems and their related options will continue until april 15, 2021. Even more colors to choose from with selectable lasers and filter replacement. Data was generated on either the bd accuri c6 plus or the bd accuri c6 as indicated in figure legends. Learn about bd life sciences diagnostic systems qcpi manuals. All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. Technology has developed, and reading accuri c6 user manual printable 2019 books could be far more convenient and simpler. Connect the cable to the socket at the back of the bd accuri c6 and switch on the bd accuri c6. For the first user of the day, press the power button to turn on cytometer.
Platforms referred to as bd accuri represent both the bd accuri c6 plus and bd accuri c6. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order. On monday morning fcrc staff perform weekly cleaning and maintenance. See the bd accuri c6 software users guide for details on instrument validation using the validation beads provided with the cytometer. Bd accuri c6 flow cytometer performance specifications summary tools. File type pdf accuri c6 user guide accuri c6 user guide bd accuri c6, flow cytometry made easy. Accuri c6 is a digital pc based bench top analyser, 2 laser system. Because the c6 flow cytometer fluidics system meters the sample fluid uptake, it is simple to obtain absolute counts, or sample concentration per microliter without the need to add beads to samples. Bd life sciences diagnostic systems qcpi manuals bd.
Free download books accuri c6 user manual printable 2019 you know that reading accuri c6 user manual printable 2019 is effective, because we could get too much info online from your reading materials. The accuri is a small, benchtop cytometer housing 2 light scatter detectors, 2 lasers, and 4 fluorescence detectors that enable the detection of up to four fluorochromes. A new workspace can be used to create an analysis template and to collect a new dataset. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Bd accuri c6 software user guide manual vi 782009501 rev0 table b1. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Bd accuri c6 plus panel builder user s guides and manuals. Do not run cells 40um in diameter the size of the flow cell is 200um and. Bd accuri c6 flow cytometer instrument manual bd biosciences.
Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Bd accuri c6 software user guide manual ii 782009501 rev0 4. Bd accuri c6 plus software files can be exported in fcs 3. Leave extra 15 min to allow the lasers to warm up note.
They prefer to invest their idle time to talk or hang out. Default cytometer configuration for the accuri c6 analyzer. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Accuri cytometers cflow user guide usf biological sciences.
It is the responsibility of the user buyer to install and maintain antivirus software on the computer. Refer to bd accuri c6 software user guide, chapter 1. The bdaccuri c6 is the flow cytometer analyzer in the flow core. Operate the instrument as described in this instruction manual. Bd has discontinued selling the bd accuri c6 flow cytometer as of december 31, 2016. Open the bead template in the bd accuri c6 software. The accuri csampler is an optional accessory for the c6 flow cytometer that. See bd accuri c6 software user s guide, chapter 1, the basics for a complete description of how to run and analyze bead results. The software for accuri c6 cflow plus is very user friendly and efficient. Accuri cytometers c6 flow cytometer community, manuals and. The intuitive interface of the software guides the user through workflows. Finally, the plant dna analysis template was opened. Using the bd accuri c6 cytometer for rapid and accurate. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs.
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